Technical Program

Paper Detail

Paper:	TH-PM-SS1.2
Session:	Computer Vision for Systems Biology
Time:	Thursday, April 6, 15:42 - 16:04
Presentation:	Special Session Oral
Title:	Time-Lapse Microscopy-Based Genome Wide RNAi Screening in Live Human Cells
Authors:	Beate Neumann; European Molecular Biology Laboratory
	Michael Held; European Molecular Biology Laboratory
	Urban Liebel; European Molecular Biology Laboratory
	Holger Erfle; European Molecular Biology Laboratory
	Phill Rogers; European Molecular Biology Laboratory
	Rainer Pepperkok; European Molecular Biology Laboratory
	Jan Ellenberg; European Molecular Biology Laboratory
Abstract:	Specific knock-down of gene expression by RNA interference is the method of choice to study gene function in human cells. A uniquely detailed phenotypic readout of such hypomorphs is possible by live cell microscopy of appropriate fluorescent reporter proteins. Here, I will present a fully automated method for RNAi screens in cultured human cells, combining reverse transfection by siRNA cellarrays, automated time-lapse fluorescence microscopy and computational phenotype analysis by image processing. The strategy is illustrated using an automatically scored mitosis assay [1] and provides an easily scalable platform that we currently use in genome-wide RNAi screens for several cellular functions.

ISBI 2006 is sponsored by

and organized in cooperation with