Technical Program

Paper Detail

Paper:	SA-PM-OS1.2
Session:	Cellular and Molecular Image Analysis
Time:	Saturday, April 8, 15:10 - 15:30
Presentation:	Oral
Title:	Multiple-Target Tracking of 3D Fluorescent Objects Based on Simulated Annealing
Authors:	Victor Racine; Institut Curie
	Ariane Hertzog; Institut Curie
	Jacqueline Jouanneau; Institut Curie
	Jean Salamero; Institut Curie
	Charles Kervrann; INRISA-INRIA
	Jean-Baptiste Sibarita; Institut Curie
Abstract:	This paper presents a framework for tracking fluorescent objects imaged with 3D+time video-microscopy. The proposed technique solves the NP-hard problem of the multi-frame object correspondence. For this purpose, we use the simulated annealing algorithm for its flexibility for feature correspondence and its ability to give results in a very short time. Our approach takes into account events like split, merge, birth and death. These evolutionary events are motivated by biological and physical considerations. We demonstrate the performance of the proposed algorithm on images of carcinoma NBT-II cells expressing fluorescent LAR-PTP, a tyrosine phosphatase possibly involved in tumor progression.

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