Technical Program

Paper Detail

Paper:	SU-AM-OS1.4
Session:	Biological Image Analysis
Time:	Sunday, April 9, 10:30 - 10:50
Presentation:	Oral
Title:	Nonuniform Temporal Alignment of Slice Sequences for Four-Dimensional Imaging of Cyclically Deforming Embryonic Structures
Authors:	Michael Liebling; California Institute of Technology
	Julien Vermot; California Institute of Technology
	Arian S. Forouhar; California Institute of Technology
	Morteza Gharib; California Institute of Technology
	Mary E. Dickinson; Baylor College of Medicine
	Scott E. Fraser; California Institute of Technology
Abstract:	The temporal alignment of nongated slice-sequences acquired at different axial positions in the living embryonic zebrafish heart permits the reconstruction of dynamic, three-dimensional data. This approach overcomes the current acquisition-speed limitation of confocal microscopes for real-time three-dimensional imaging of fast processes. Current synchronization methods align and uniformly scale the data in time, but do not compensate for slight variations in the heart rhythm that occur within a heartbeat. Therefore, they impose constraints on the admissible data quality. Here, we derive a nonuniform registration procedure based on the minimization of the absolute value of the intensity difference between adjacent slice-sequence pairs. The method compensates for temporal intra-sample variations and allows the processing of a wider range of data to build functional, dynamic models of the beating embryonic heart. We show reconstructions from data acquired in living, fluorescent zebrafish embryos.

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