Paper: | TH-PM-SS1.2 |
Session: | Computer Vision for Systems Biology |
Time: | Thursday, April 6, 15:42 - 16:04 |
Presentation: |
Special Session Oral
|
Title: |
Time-Lapse Microscopy-Based Genome Wide RNAi Screening in Live Human Cells |
Authors: |
Beate Neumann; European Molecular Biology Laboratory | | |
| Michael Held; European Molecular Biology Laboratory | | |
| Urban Liebel; European Molecular Biology Laboratory | | |
| Holger Erfle; European Molecular Biology Laboratory | | |
| Phill Rogers; European Molecular Biology Laboratory | | |
| Rainer Pepperkok; European Molecular Biology Laboratory | | |
| Jan Ellenberg; European Molecular Biology Laboratory | | |
Abstract: |
Specific knock-down of gene expression by RNA interference is the method of choice to study gene function in human cells. A uniquely detailed phenotypic readout of such hypomorphs is possible by live cell microscopy of appropriate fluorescent reporter proteins. Here, I will present a fully automated method for RNAi screens in cultured human cells, combining reverse transfection by siRNA cellarrays, automated time-lapse fluorescence microscopy and computational phenotype analysis by image processing. The strategy is illustrated using an automatically scored mitosis assay [1] and provides an easily scalable platform that we currently use in genome-wide RNAi screens for several cellular functions. |