Technical Program

Paper Detail

Paper:	SA-PM-OS1.7
Session:	Cellular and Molecular Image Analysis
Time:	Saturday, April 8, 17:20 - 17:40
Presentation:	Oral
Title:	Quantitative Cell Motility for In Vitro Wound Healing Using Level Set-Based Active Contour Tracking
Authors:	Filiz Bunyak; University of Missouri-Columbia
	Kannappan Palaniappan; University of Missouri-Columbia
	Sumit K. Nath; University of Missouri-Columbia
	Tobias I. Baskin; University of Massachusetts
	Gang Dong; University of Massachusetts
Abstract:	Quantifying the behavior of cells individually, and in clusters as part of a population, under a range of experimental conditions, is a challenging computational task with many biological applications. We propose a versatile algorithm for segmentation and tracking of multiple motile epithelial cells during wound healing using time-lapse video. The segmentation part of the proposed method relies on a level set-based active contour algorithm that robustly handles a large number of cells. The tracking part relies on a detection-based multiple-object tracking method with delayed decision enabled by multi-hypothesis testing. The combined method is robust to complex cell behavior including division and apoptosis, and to imaging artifacts such as illumination changes.

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