T-4: Advanced optical microscopy: challenges and opportunities

Date: Wednesday, May 14

Presented by

Dr. Rainer Heinzmann, King’s College London, Randall Division, London, United Kingdom

Abstract

This tutorial focuses on high-resolution and multispectral fluorescence imaging and the associated image reconstruction techniques. A range of recent developments will be covered, most of which would not be possible without the help of extensive signal processing. Pitfalls of the respective methods posing challenges to the image processing will be stressed in each case.

The goal of this tutorial is to introduce engineers to current day fluorescence microscopy technology and sharpen their senses as to which methods may be useful for what type of bioimaging questions.

The first part will deal with methods which do not essentially rely on image processing, but processing can enhance their image quality, while the second will concentrate on methods which have only become possible through the help of image processing.

Part 1: Direct Methods

• Confocal microscopy, 4Pi microscopy and Stimulated Emission Depletion (STED)
• Efficient Interferometric Confocal Signal Detection
• The Programmable Array Microscope (PAM)
• Weighted Fourier-Space Averaging and Multiple-View Deconvolution

Part 2: Indirect Methods

• Imaging by Localization of Particles (Pointillism, PALM, STORM, PALMIRA)
• Multispectral Excitation and Emission Imaging using Fourier Encoding
• Structured Illumination and Image Reconstruction by Fourier-Synthesis

Speaker Biography

Rainer Heintzmann received the diploma in Physics at the University of Osnabrueck in 1996 and in 1999 his Ph.D. in Physics from the University of Heidelberg, Germany. From 2000 to 2004, he worked as a scientist in the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany. He now heads the Biological Nanoimaging Group at the Randall Division of Cell and Molecular Biophysics at King’s College London. His main research area is high-resolution fluorescence microscopy. He has contributed to the development of many of the above mentioned imaging modes. He also has a strong interest in various areas of image processing and is the author of over 30 published journal papers in biomedical imaging.